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[fifty nine] 2nd, measured assay concentrations fail to become consistent because cTnI circulates in various kinds; the antibodies used in the obtainable assays understand different epitopes of cTnI even for assays and devices marketed by a similar maker.[60]
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cTnI in the sample results in being labeled with an ALP-conjugated antibody and is captured onto the sensor surface throughout the incubation stage.[forty nine] Clean fluid made up of enzyme substrate slot dana is then placed on get rid of unbound substances, and simultaneously, ALP certain to the antigen-antibody sophisticated reacts, releasing an electrochemically detectable solution. The produced amperometric signal is directly proportional to the topanwin login cTnI from the sample.[50]
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